Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens

The present investigation was carried out to study the active component of Bacillus subtilis, a gastrointestinal bacterium of mrigal (Cirrhinus mrigala) that shows inhibitory activity against a wide range of fish pathogens. The bacterium was found to inhibit all the fish pathogens and an extra cellular protein fraction (ECP) of 5-10 kD was found to be the active component responsible for inhibitory activity. The component was heat stable (60°C for 1 h) and a change in pH had no effect on the antibacterial activity. The protein fraction was sensitive to trypsin, chymotrypsin, lysozyme, and proteinase K at 2.5 mg/ml and resistant at 0.1 and 0.5 mg/ml. The present investigation confirmed that the active component of the B. subtilis responsible for antibacterial activities against common fish pathogens is a low molecular weight protein fraction (5-10 kD). A protein or peptide with such a low molecular weight has the potential to serve as an alternative health management strategy for combating disease in aquaculture

Pathology of mixed infections of saprolegniasis-myxosporidiosis in Indian major carp (Catla catla Ham.)

An outbreak of saprolegniasis in Catla catla in composite carp culture ponds were recorded during winter season. The typical cotton wool growths were observed on whole body surfaces of catla along with sporadic mortality. The fungal invasion was only restricted to skin and no fungal elements were visible in any internal organs after periodic acid schiff staining. On histology, periportal accumulation of mononuclear cells in liver, presence of myxosporidean cysts in antieror kidney, eosinophilic granular cells reaction in submucosa of stomach and intestine, dilated and engorged blood vessels of brain along with sloughing of epidermis and hyperplasia at gill lamellar base were pronounced changes. The possible role of release of Saprolegnia toxin in producing internal organs pathology has been discussed

The STAR Photon Multiplicity Detector

Details concerning the design, fabrication and performance of STAR Photon Multiplicity Detector (PMD) are presented. The PMD will cover the forward region, within the pseudorapidity range 2.3--3.5, behind the forward time projection chamber. It will measure the spatial distribution of photons in order to study collective flow, fluctuation and chiral symmetry restoration.Comment: 15 pages, including 11 figures; to appear in a special NIM volume dedicated to the accelerator and detectors at RHI

Point defect induced incommensurate dipole moments in the KCa2Nb3O10 Dion-Jacobson layered perovskite

Local structural distortions due to isolated atomic defects and defect complexes strongly affect the macroscopic properties of oxide ceramics. While the characterization of local defect structures is more common in simple ABO3 perovskites, unambiguous determination of the same in layered perovskites is more difficult due to their complex crystal structures. Here, we combined x-ray pair distribution function and density functional theory calculations to characterize the structure of cation-oxygen divacancy pairs in a Dion-Jacobson (D-J) layered perovskite. Our results indicate that local incommensurate dipole moments with polarization density in the range of ∼0.1–17μC/cm2 are created due to divacancy-induced structural distortions in the D-J phase KCa2Nb3O10. This is comparable with defect dipole moments observed in well-known perovskite ferroelectrics. The current results imply that controlling the atomic defects can potentially lead to significant control of dielectric properties in D-J layered perovskites

Antibacterial and Morphological Studies of Electrospun Silver-Impregnated Polyacrylonitrile Nanofibre

Silver-impregnated polyacrylonitrile (PAN) nanofibre was prepared through electrospinning process. Infra-red spectroscopy (FT-IR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Thermo gravimetric analyses (TGA/DTA) were used to characterize PAN and PAN/Ag composites. The XRD results reflects decrease in the crystallinity of PAN as it is been modified with Ag. Antibacterial activity of PAN/Ag was also investigated

A Honeycomb Proportional Counter for Photon Multiplicity Measurement in the ALICE Experiment

A honeycomb detector consisting of a matrix of 96 closely packed hexagonal cells, each working as a proportional counter with a wire readout, was fabricated and tested at the CERN PS. The cell depth and the radial dimensions of the cell were small, in the range of 5-10 mm. The appropriate cell design was arrived at using GARFIELD simulations. Two geometries are described illustrating the effect of field shaping. The charged particle detection efficiency and the preshower characteristics have been studied using pion and electron beams. Average charged particle detection efficiency was found to be 98%, which is almost uniform within the cell volume and also within the array. The preshower data show that the transverse size of the shower is in close agreement with the results of simulations for a range of energies and converter thicknesses.Comment: To be published in NIM

Surface Incompressibility from Semiclassical Relativistic Mean Field Calculations

By using the scaling method and the Thomas-Fermi and Extended Thomas-Fermi approaches to Relativistic Mean Field Theory the surface contribution to the leptodermous expansion of the finite nuclei incompressibility has been self-consistently computed. The validity of the simplest expansion, which contains volume, volume-symmetry, surface and Coulomb terms, is examined by comparing it with self-consistent results of the finite nuclei incompressibility for some currently used non-linear sigma-omega parameter sets. A numerical estimate of higher-order contributions to the leptodermous expansion, namely the curvature and surface-symmetry terms, is made.Comment: 18 pages, REVTeX, 3 eps figures, changed conten

Genetic Dissection of Drought and Heat Tolerance in Chickpea through Genome-Wide and Candidate Gene-Based Association Mapping Approaches

To understand the genetic basis of tolerance to drought and heat stresses in chickpea, a comprehensive association mapping approach has been undertaken. Phenotypic data were generated on the reference set (300 accessions, including 211 mini-core collection accessions) for drought tolerance related root traits, heat tolerance, yield and yield component traits from 1–7 seasons and 1–3 locations in India (Patancheru, Kanpur, Bangalore) and three locations in Africa (Nairobi, Egerton in Kenya and Debre Zeit in Ethiopia). Diversity Array Technology (DArT) markers equally distributed across chickpea genome were used to determine population structure and three sub-populations were identified using admixture model in STRUCTURE. The pairwise linkage disequilibrium (LD) estimated using the squared-allele frequency correlations (r2; when r2<0.20) was found to decay rapidly with the genetic distance of 5 cM. For establishing marker-trait associations (MTAs), both genome-wide and candidate gene-sequencing based association mapping approaches were conducted using 1,872 markers (1,072 DArTs, 651 single nucleotide polymorphisms [SNPs], 113 gene-based SNPs and 36 simple sequence repeats [SSRs]) and phenotyping data mentioned above employing mixed linear model (MLM) analysis with optimum compression with P3D method and kinship matrix. As a result, 312 significant MTAs were identified and a maximum number of MTAs (70) was identified for 100-seed weight. A total of 18 SNPs from 5 genes (ERECTA, 11 SNPs; ASR, 4 SNPs; DREB, 1 SNP; CAP2 promoter, 1 SNP and AMDH, 1SNP) were significantly associated with different traits. This study provides significant MTAs for drought and heat tolerance in chickpea that can be used, after validation, in molecular breeding for developing superior varieties with enhanced drought and heat tolerance

Search for DCC in 158A GeV Pb+Pb Collisions

A detailed analysis of the phase space distributions of charged particles and photons have been carried out using two independent methods. The results indicate the presence of nonstatistical fluctuations in localized regions of phase space.Comment: Talk at the PANIC99 Conference, June 9-16, 199